Server Input
is-pero is a web server designed for the prediction and classification of peroxisomal proteins. It utilizes advanced machine learning models and sequence analysis techniques to provide accurate and interpretable results. The server supports high-throughput proteomics studies by allowing batch predictions.
is-pero accepts input in the form of protein sequences provided in FASTA format. Users can submit up to 100 protein sequences per job. These sequences can either be pasted directly into the input box or uploaded as a FASTA file.
MPTS Prediction
The server provides an optional MPTS analysis, which uses embeddings to predict potential membrane peptide signal regions (MPTS). This option enhances the classification of membrane-associated peroxisomal proteins by evaluating PEX19-binding sites.
Server Output
Upon submission, is-pero processes the input and returns a downloadable CSV file containing detailed results. The output includes sequence IDs, localization probabilities, signal peptide matches, and predictions for MPTS regions if selected. This structured output allows easy interpretation and integration into further proteomics workflows.
Usage Notes
Ensure that all input sequences are in the correct FASTA format. Jobs may take longer to process if the maximum number of sequences (100) is submitted. For best results, review the MPTS predictions alongside other localization.
Contact
For additional support or inquiries, please contact the development team at marco.anteghini2@unibo.it.